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Zhen Dong is a Research Assistant Professor at the Westlake Laboratory of Life Sciences and Biomedicine. He obtained his PhD from the University of Otago, New Zealand, in December 2020. From 2021 to 2023, he conducted postdoctoral research in Guomics at Westlake University, focusing on the development and application of expansion-based spatial proteomics.

Over the past five years, Zhen has received several grants and awards, including the International Postdoctoral Exchange Fellowship (Introduction Program), the Westlake Laboratory “Frontier Scholar”, the Special Postdoctoral Research Grant, the Rising Star Project from the National Key Laboratory of Medical Proteomics, and the π-HuB Rising Star Award. He has also contributed to multiple national key projects supported by the National Natural Science Foundation of China.

Zhen has published 12 papers as first author (including co-first) or corresponding author (including co-corresponding) in journals such as Nature Communications, Nature Protocols, Hepatology, Trends in Biochemical Sciences, and Cell Discovery. He co-developed the first-generation expansion spatial proteomics technology, ProteomEx, and led the development of its second-generation method, FAXP, which were featured by Nature in its 2025 “Technology Features” series as major advances in spatial proteomics and recognized by Trends in Biochemical Sciences as the “Technology of the Month.” More information can be found at guomics.com/faxp.

Representative Publications

[Note: *, co-corresponding; ^#, co-first author]

1. Zhen Dong#, Chunlong Wu#, Jiayi Chen, Wenhao Jiang, Kiryl D. Piatkevich*, Yi Zhu*, Tiannan Guo*. Filter-aided expansion proteomics for the spatial analysis of single cells and organelles in FFPE tissue samples. Nature Protocols. 2025 Oct 9:1-25.

2. Zhang, H.#, L. Hong#, Dong#, S. Xin#, B. Lin, J. Cheng, W. Tian, B. Li, J. Wang, X. Liu, C. Liu, Y. Jin, Y. Feng, G. Su, X. Sun, Q. Liu, X. Dai, Y. Gao, Z. Tong, L. Liu, X. Zhu, Y. Zheng, P. Zhao, T. Guo*, W. Fang* and X. Bao*. Spatially resolved C1QC+ macrophage-CD4+ T cell niche in colorectal cancer microenvironment: implications for immunotherapy response. Cell Discovery. 2025 11(1): 60. https://doi.org/10.1038/s41421-025-00811-2

3. Zhen Dong#, Wenhao Jiang#, Tiannan Guo*. Tissue expansion enables proteomics at improved spatial resolution. Trends in Biochemical Sciences. 2025 June 21. doi: 10.1016/j.tibs.2025.06.002

4. Liujia Qian, Zhen Dong & Tiannan Guo*. Grow AI Virtual Cells: Three Data Pillars and Closed-Loop Learning. Cell Research. 2025 35: 319-321. https://doi.org/10.1038/s41422-025-01101-y

5. Libing Hong#, Jie Mei#, Xuqi Sun#, Yifan Wu#, Zhen Dong#, Yuzhi Jin, Liaoliao Gao, Jinlin Cheng, Weihong Tian, Chuan Liu, Bin Li, Pingping Hu, Lulu Liu, Shan Xin, Xiaomeng Dai, Peng Zhao, Rongping Guo, Minshan Chen, Jingping Yun, Bo Lin*, Wei Wei*, Weijia Fang*, Xuanwen Bao*. Spatial single-cell proteomics landscape decodes the tumor microenvironmental ecosystem of intrahepatic cholangiocarcinoma. Hepatology. 2025 Feb 25. doi: 10.1097/HEP.0000000000001283

6. Zhen Dong#, Wenhao Jiang#, Chunlong Wu, Ting Chen, Jiayi Chen, Xuan Ding, Shu Zheng, Kiryl D Piatkevich*, Yi Zhu*, Tiannan Guo*. Spatial proteomics of single cells and organelles on tissue slides using filter-aided expansion proteomics. Nature Communications. 2024 Oct 30;15(1):9378. doi: 10.1038/s41467-024-53683-7

7. Hu C#, Hu W#, Tang B#, Bao Q#, Jiang X#, Tang L, Wang H, He L, Lv M, Xiao Y, Liu C, Li X, Liu Y, Li J, Huang G, Dong Z*, Li Z*, Guo T*, Yang S*. Plasma and urine proteomics and gut microbiota analysis reveal potential factors affecting COVID-19 vaccination response. iScience. 2024 Jan 8;27(2):108851.

8. Lu Li#, Cuiji Sun#, Yaoting Sun#, Zhen Dong#, Runxin Wu, Xiaoting Sun, Hanbin Zhang, Wenhao Jiang, Yan Zhou, Xufeng Cen, Shang Cai, Hongguang Xia, Yi Zhu, Tiannan Guo* & Kiryl D. Piatkevich*. Spatially resolved proteomics via tissue expansion. Nature Communications. 2022.13: 7242.

Oral talks

1.  Oct 15–17, 2025

23rd Annual Conference of the Korean Human Proteome Organization (KHUPO 2025), Busan, South Korea – Invited Session Talk

2. Oct 12–14, 2025

Joint 12th AOHUPO, 8th AOAPO, and 3rd π-HuB Global Summit in conjunction with the 13th CNHUPO Congress, Guangzhou, China – Oral Presentation (Rising Star Symposium)

3. Jul 2–3, 2025

The First Symposium on Single-Cell Proteomics, Hangzhou, China – Invited Talk

4. Oct 20–24, 2024

HUPO 2024 World Congress, Dresden, Germany – Oral Presentation

5. Sep 17–21, 2023

HUPO 2023 World Congress, Busan, South Korea – Oral Presentation

Society

1. Jul 2025 – present

Guest Editor, “High-Resolution Spatial Proteomics at Single-Cell Levels” Collection, Clinical Proteomics

2. Dec 2024 – present

Member, Single Cell Proteomics Initiative (HUPO)